Web Design
AVC-Logo
UPEI square logo_200
VPm 2010 - 2017
 
 
LAB 8
Urinary Tract Infections

 

Laboratory exercises

 

#1. You are provided with a canine urine sample collected using cystocentesis. Inoculate BA plates using the quantitative plating technique (see Section C3a.). Using plastic-disposable loops, one student will plate 10.0 ul & one will plate 1.0 ul. Include the volumes on your plate labels.

    a. BA results: one type of colonies, double zone hemolysis

    b.Colony counts & conversion to bacteria/mL (of urine):

      Bacteria/mL for 1.0 uL vol = colony number x 1000. __________

      Bacteria/mL for 10.0 uL vol = colony number x 100. __________

    Consult the Table below - does this patient have an infection? Yes

 

Staph.intermedius_complete-hemolysis1 wb  Urine-quan-BA-2 wb staph aureus wb

Staphylococcus pseudintermedius
left BA showing double zone hemolysis;
right BA showing  quantitative plate that allows bacterial colony counts to be carried out.

 

 

    Method of Collection

    Results indicative of infection*

    not infection

    Cystocentesis

    >1,000a

    <1000

    Catheterization

    >10,000 in males

    <1,000 in males, higher in females

    Voided

    cannot determine in dogs

    <10,000

    * Number of bacteria bacteria/ml of urine
    a
    Basically any bacteria in bladder suggest infection, skin or colon/fecal contaminants are a consideration

 

#2. You are provided with urine samples collected by cystocentesis from cats with a recurring history of inappropriate urination. Samples are labelled A, B or C.

Using the quantitative plating technique plate 10.0 uL’s of urine on BA and MAC plates. Label your plates properly.

 

 

quantitative plating technique

 

 

Do a Gram stain and examine under oil. Record your results.

 

      A. E.coli

      Recall E.coli is a Lactose Fermenter on MacConkey.

UTI E.coli

 
Ecoli-culture-Gstain-05 wb
 

       

      B. Staphylococcus felis

      Hemolytic, coagulase negative (See Demo.3)

staph epidermidis wb

       

      C. Enterococcus faecalis

UTI  E faecalis

      non-hemolytic, Gram positive cocci, esculin positive

 

 

cytocentesis
Cystocentesis - art by Kip Carter  2005 Univ. Georgia Res.
Foundation Inc. Greene: Infectious Diseases of the Dog & Cat, 4th Ed. , 2012.

 

[8A exercises]